AI Article Synopsis
- A study investigated the anti-diabetic and anti-urease properties of ONL leaves, identifying six active compounds including quercetin-3-O-glucoside and myricetin.
- The butanol sub-fraction BE2 showed strong inhibition of both α-glucosidase and Jack bean urease, outperforming the standard drugs acarbose and thiourea.
- BE2 significantly increased glucose uptake in treated cells, suggesting its effectiveness at lower concentrations than the ethyl acetate sub-fraction EA5, with further research needed to explore its full potential.
Article Abstract
A study was conducted to investigate the anti-diabetic and anti-urease potential of leaves (ONL). Six compounds, i.e. quercetin-3-O-glucoside, myricetin, shikimic acid, catechin, trans-ferulic acid and luteolin were identified from the butanol sub-fraction, BE2 and the ethyl acetate sub-fraction, EA5 of ONL. BE2 inhibited α-glucosidase and Jack bean urease with IC values of 0.036 μg/mL (437.46 μg/mL for acarbose) and 0.327 mg/mL (0.039 mg/mL for thiourea), respectively. In the glucose uptake experiment, BE2 (0.05 mg/mL) treatment resulted in a substantial increase in glucose uptake in free fatty acid (FFA)-treated cells at a concentration 10 times lower than that seen in EA5 (0.5 mg/mL) treated cells. The binding energies of quercetin-3-O-glucoside with α-glucosidase, glucose transporter GLUT4 and urease were found to be -94.2585, -219.8271 and -254.391 kcal/mol, respectively. This study revealed that ONL has anti-diabetic and anti-urease abilities and further in-depth research may unveil its full potential.
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| http://dx.doi.org/10.1080/14786419.2023.2265039 | DOI Listing |
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Article Synopsis
- A study investigated the anti-diabetic and anti-urease properties of ONL leaves, identifying six active compounds including quercetin-3-O-glucoside and myricetin.
- The butanol sub-fraction BE2 showed strong inhibition of both α-glucosidase and Jack bean urease, outperforming the standard drugs acarbose and thiourea.
- BE2 significantly increased glucose uptake in treated cells, suggesting its effectiveness at lower concentrations than the ethyl acetate sub-fraction EA5, with further research needed to explore its full potential.
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