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A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy.
J Biol Eng
January 2025
AVT - Biochemical Engineering, RWTH Aachen University, Forckenbeckstr. 51, D-52074, Aachen, Germany.
Background: Shake flasks are essential tools in biotechnological development due to their cost efficiency and ease of use. However, a significant challenge is the miniaturization of process analytical tools to maximize information output from each cultivation. This study aimed to develop a respiration activity online measurement system via off-gas analysis, named "Transfer rate Online Measurement" (TOM), for determining the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and the respiration quotient (RQ) in surface-aerated bioreactors, primarily targeting shake flasks.
View Article and Find Full Text PDFGuided Self-Help vs Group Treatment for Children With Obesity: A Randomized Clinical Trial.
Pediatrics
January 2025
Department of Psychiatry, University of Minnesota, Minneapolis, Minnesota.
Background And Objectives: Family-based behavioral treatment (FBT) for children with obesity is provided in weekly parent and child groups over 6 months. A guided self-help FBT program (gshFBT) is provided to the dyad in short meetings. Both interventions provide the same content; however, gshFBT provides this content in less time (FBT = 23 hours, gshFBT = 5.
View Article and Find Full Text PDFMarigold: a machine learning-based web app for zebrafish pose tracking.
BMC Bioinformatics
January 2025
Biology Department, University of Massachusetts Amherst, Amherst, MA, USA.
Background: High-throughput behavioral analysis is important for drug discovery, toxicological studies, and the modeling of neurological disorders such as autism and epilepsy. Zebrafish embryos and larvae are ideal for such applications because they are spawned in large clutches, develop rapidly, feature a relatively simple nervous system, and have orthologs to many human disease genes. However, existing software for video-based behavioral analysis can be incompatible with recordings that contain dynamic backgrounds or foreign objects, lack support for multiwell formats, require expensive hardware, and/or demand considerable programming expertise.
View Article and Find Full Text PDFAssessing the performance of AI chatbots in answering patients' common questions about low back pain.
Ann Rheum Dis
January 2025
Masters and Doctoral Programs in Physical Therapy, Universidade Cidade de Sao Paulo, Sao Paulo, Brazil; Discipline of Physiotherapy, Graduate School of Health, Faculty of Health, University of Technology, Sydney, New South Wales, Australia.
Objectives: The aim of this study was to assess the accuracy and readability of the answers generated by large language model (LLM)-chatbots to common patient questions about low back pain (LBP).
Methods: This cross-sectional study analysed responses to 30 LBP-related questions, covering self-management, risk factors and treatment. The questions were developed by experienced clinicians and researchers and were piloted with a group of consumer representatives with lived experience of LBP.
Assessing Preferences of Patients with Chronic Spontaneous Urticaria for Injectable Treatment Profiles.
Patient
January 2025
Division of Rheumatology, Allergy and Immunology, Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Background: In the context of injectable biologic products approved or in development for chronic spontaneous urticaria (CSU), it is important to capture which treatment attributes matter most to patient and what trade-offs patients are willing to make.
Objectives: The CHOICE-CSU study aimed to quantify patient preferences toward injectable treatment attributes among patients with CSU, inadequately controlled by H1-antihistamines.
Methods: This was a two-phase cross-sectional patient preference study in adult patients with a diagnosis of CSU, inadequately controlled by H1-antihistamines.
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