The Effect of Aqueous Extract on TM3 Leydig Cells Exposed to TNF-α .

Background: Extractions of () are shown to have immune modulation, anti-inflammatory and antioxidant properties. However, is also cytotoxic, antiproliferative, and pro-apoptotic . Furthermore, extractions may influence steroidogenesis. Nevertheless, the impact on Leydig cell function has not previously been investigated. As tumor necrosis factor-alpha (TNF-α) is known to cause Leydig cell dysfunction under inflammatory conditions, it is further proposed that extracts may protect against the negative impact of TNF-α on Leydig cells. The aim of the study was to investigate the effect of an aqueous extract () on Leydig cells exposed to TNF-α.

Methods: Human chorionic gonadotrophin-stimulated TM3 Leydig cells were exposed for 24 h to (a) TNF-α (0.1, 1, 10, 100 ng/mL), (b) (0.01, 0.1, 1, 10, 100 ng/mL), and (c) co-exposure to 10 ng/mL TNF-α and (0.01, 0.1, 1, 10, 100 ng/mL). We analyzed cell viability, cytotoxicity, caspase 3/7 activation, testosterone concentration, and intracellular superoxide.

Results: TNF-α exposure decreased cell viability, increased cytotoxicity, and caspase 3/7, with no significant effect on intracellular superoxide in TM3 Leydig cells. When concentrations of 0.01-10 ng/mL were tested, we observed improved vitality and reduced levels of caspase 3/7. At 100 ng/mL, decreased viability and increased cytotoxicity and caspase 3/7. However, did not affect intracellular superoxide. Furthermore, protected against 10 ng/mL TNF-α-induced cytotoxicity and apoptosis, except at the highest concentration. alone and in co-culture with 10 ng/mL TNF-α increased testosterone at high concentrations.

Conclusions: In our TM3 Leydig cell model, protected against TNF-α-induced cytotoxicity and early apoptosis, except at the highest experimental concentrations, where it was cytotoxic. These effects were not mediated through a change in intracellular superoxide. Although further investigations are warranted, aqueous may protect against TNF-α-induced Leydig cell dysfunction.