Six psychrotolerant, Gram-stain-negative, aerobic bacterial strains, designated as LB1P51, LB2P87, LB2P84, LB3P48, LB3R18 and XS2P67, were isolated from glaciers on the Tibetan Plateau, PR China. The results of 16S rRNA gene analysis confirmed their classification within the genus . Strain LB2P87 displayed the highest sequence similarity to 0533 (98.18 %), while strain LB1P51 exhibited the highest sequence similarity to CGMCC 1.5380 (98.15 %). Strains LB2P87 and LB1P51 had genome sizes of 3.8 and 3.9 Mb, respectively, with DNA G+C contents of 34.2 and 34.1 %, respectively. Pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) calculations revealed that these strains represented two distinct species within the genus . The results of phylogenomic analysis using 606 core genes indicated that the six strains formed a distinct clade and were most closely related to CGMCC 1.5380. The ANI and dDDH values between the two species and other members of the genus were below 90.3 and 40.1 %, respectively. Genome relatedness, the results of phylogenomic analysis and phenotypic characteristics collectively support the proposal of two novel species of the genus : sp. nov. (LB1P51 = CGMCC 1.11237 = NBRC 114813) and sp. nov. (LB2P87 = CGMCC 1.11249 = NBRC 114814).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/ijsem.0.006072 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cross-protectivity of henipavirus soluble glycoprotein in an model of Nipah virus disease.
Front Immunol
March 2025
Specialised Microbiology and Laboratories, United Kingdom Health Security Agency (UKHSA), Salisbury, Wiltshire, United Kingdom.
Introduction: Nipah virus (NiV) is one of a group of highly pathogenic viruses classified within the Henipavirus genus. Since 2012 at least 11 new henipa-like viruses have been identified, including from new locations and reservoir hosts; the pathogenicity of these new viruses has yet to be determined, but two of them have been associated with morbidity, including fatalities.
Methods: The efficacy and cross-reactivity of two vaccine candidates derived from the soluble glycoproteins of both NiV and Hendra virus (HeV) was evaluated in our recently established hamster model.
Functional analysis and interaction networks of Rboh in poplar under abiotic stress.
Front Plant Sci
February 2025
Key Laboratory for Forest Resource Conservation and Utilization in the Southwest Mountains of China (Ministry of Education), College of Forestry, Southwest Forestry University, Kunming, China.
Introduction: Plant respiratory burst oxidase homologs (Rbohs) are essential in the generation of reactive oxygen species (ROS) and play critical roles in plant stress responses. Despite their importance, Rbohs in poplar species remain under-explored, especially in terms of their characteristics and functional diversity across different species within the same genus.
Methods: In this study, we employed bioinformatics methods to identify 62 Rboh genes across five poplar species.
A phoenix in the greenhouse: characterization and phylogenomics of complete chloroplast genomes sheds light on the putatively extinct-in-the-wild Solanum ensifolium (Solanaceae).
BMC Plant Biol
March 2025
Department of Biology, Eastern Connecticut State University, Willimantic, CT, 06226, USA.
Background: The genus Solanum is a diverse group of flowering plants with significant economic importance. Within this genus, the subgenus Leptostemonum, comprising spiny solanums, is particularly noteworthy due to its high species diversity and endemism. Solanum ensifolium, a member of this subgenus, is a critically endangered species endemic to Puerto Rico and known locally as erubia.
View Article and Find Full Text PDFgen. nov., sp. nov., a novel halophilic bacterium isolated from tidal flat sediment.
Int J Syst Evol Microbiol
March 2025
Department of Integrative Biotechnology, Sungkyunkwan University, Natural Science Campus, 2066 Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do 16419, Republic of Korea.
A novel bacterial strain, MJW-29, was isolated from tidal flat sediment in Gochang, Republic of Korea. The isolate is Gram-stain-negative, facultatively anaerobic, gliding motile and short rods. The strain MJW-29 is oxidase and catalase positive.
View Article and Find Full Text PDFComplete genome sequence of fig leaf mottle-associated virus 2.
Arch Virol
March 2025
Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
The complete genome sequence of fig leaf mottle-associated virus 2 (FLMaV2), a positive-sense single-stranded RNA virus with a genome length of 16,925 nucleotides, was determined via RT-PCR and rapid amplification of cDNA ends (RACE). Its genome organization resembles those of little cherry virus 2 (LChV2) and yam asymptomatic virus 1 (YaV1), both of which are currently classified as members of the genus Ampelovirus but differ from typical ampeloviruses. Phylogenetic analysis based on amino acid sequences of the RNA-dependent RNA polymerase and coat protein of members of the family Closteroviridae showed that FLMaV2 clustered with LChV2 and YaV1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!