AI Article Synopsis

  • Obesity leads to vascular dysfunction and chronic inflammation in adipose tissue, promoting a process called endothelial-to-mesenchymal transition (EndoMT), where endothelial cells become pro-inflammatory and fibrotic mesenchymal-like cells.
  • In a study using mice, it was found that a high-fat/high-sucrose diet significantly increases EndoMT over time in both subcutaneous and visceral adipose tissues, with flow cytometry revealing a marked rise in mesenchymal-like cells.
  • Further analysis, including transcriptomic studies of human cells and single-cell RNA datasets from obese individuals, confirmed the association of EndoMT with inflammatory signaling pathways, indicating a potential mechanism linking obesity to vascular and tissue dysfunction.

Article Abstract

Introduction: Vascular dysfunction and chronic inflammation are characteristics of obesity-induced adipose tissue dysfunction. Proinflammatory cytokines can drive an endothelial-to-mesenchymal transition (EndoMT), where endothelial cells undergo a phenotypic switch to mesenchymal-like cells that are pro-inflammatory and pro-fibrotic. In this study, we sought to determine whether obesity can promote EndoMT in adipose tissue.

Methods: Mice in which endothelial cells are lineage-traced with eYFP were fed a high-fat/high-sucrose (HF/HS) or Control diet for 13, 26, and 52 weeks, and EndoMT was assessed in adipose tissue depots as percentage of CD45CD31Acta2 mesenchymal-like cells that were eYFP . EndoMT was also assessed in human adipose endothelial cells through cell culture assays and by the analysis of single cell RNA sequencing datasets obtained from the visceral adipose tissues of obese individuals.

Results: Quantification by flow cytometry showed that mice fed a HF/HS diet display a time-dependent increase in EndoMT over Control diet in subcutaneous adipose tissue (+3.0%, +2.6-fold at 13 weeks; +10.6%, +3.2-fold at 26 weeks; +11.8%, +2.9-fold at 52 weeks) and visceral adipose tissue (+5.5%, +2.3-fold at 13 weeks; +20.7%, +4.3-fold at 26 weeks; +25.7%, +4.8-fold at 52 weeks). Transcriptomic analysis revealed that EndoMT cells in visceral adipose tissue have enriched expression of genes associated with inflammatory and TGFβ signaling pathways. Human adipose-derived microvascular endothelial cells cultured with TGF-β1, IFN-γ, and TNF-α exhibited a similar upregulation of EndoMT markers and induction of inflammatory response pathways. Analysis of single cell RNA sequencing datasets from visceral adipose tissue of obese patients revealed a nascent EndoMT sub-cluster of endothelial cells with reduced and increased expression, which was also enriched for inflammatory signaling genes and other genes associated with EndoMT.

Discussion: These experimental and clinical findings show that chronic obesity can accelerate EndoMT in adipose tissue. We speculate that EndoMT is a feature of adipose tissue dysfunction that contributes to local inflammation and the systemic metabolic effects of obesity..

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10546194PMC
http://dx.doi.org/10.3389/fcvm.2023.1264479DOI Listing

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