Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential.

This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO and leaf extract of as the precursor material. In order to maximize the antibacterial efficacy against , , and , the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against , , and , and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs.Communicated by Ramaswamy H. Sarma.