AI Article Synopsis
- Research on alcohol dehydrogenase (ADH) in young soybean seedlings showed that specific activity is high in radicle tissue but decreases as roots develop, becoming nearly undetectable with the emergence of true leaves.
- Treatment with 2,4-Dichlorophenoxyacetic acid (2,4-D) significantly boosted ADH specific activity, increasing it by up to 14 times.
- Only one ADH isozyme was identified, and DNA analysis revealed some genetic similarities between soybean ADH and maize ADH1 cDNA, suggesting the potential presence of multiple Adh genes in soybeans.
Article Abstract
Molecular properties of alcohol dehydrogenase (ADH) were examined in young soybean seedlings. Soybean radicle tissue is ADH-rich. Enzyme specific activity decreases slowly with the development of roots and becomes almost undetectable when the first true leaves appear. Soybean ADH was not found to be inducible by flooding. 2,4-Dichlorophenoxyacetic acid (2,4-D) treatment increased ADH specific activity as much as 14-fold. Only one ADH isozyme was detected by isoelectric focusing. By DNA-DNA hydridization, soybean ADH genomic sequences were shown to be partly homologous to maize ADH1 cDNA. The presence of more than one Adh gene in soybean is discussed.
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| http://dx.doi.org/10.1007/BF00498999 | DOI Listing |
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