Unlabelled: Molecular oxygen (O ) is one of the most functionally relevant metabolites. O is essential for mito-chondrial aerobic respiration. Changes in O affect muscle metabolism and play a critical role in the maintenance of skeletal muscle mass, with lack of sufficient O resulting in detrimental loss of muscle mass and function. How exactly O is used by muscle cells is less known, mainly due to the lack of tools to address O dynamics at the cellular level. Here we discuss a new imaging method for the real time quantification of intracellular O in muscle cells based on a genetically encoded O -responsive sensor, Myoglobin-mCherry. We show that we can spatially resolve and quantify intracellular O concentration in single muscle cells and that the spatiotemporal O gradient measured by the sensor is linked to, and reflects, functional metabolic changes occurring during the process of muscle differentiation.
Highlights: Real time quantitation of intracellular oxygen with spatial resolutionIdentification of metabolically active sites in single cellsOxygen metabolism is linked to muscle differentiation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541121 | PMC |
| http://dx.doi.org/10.1101/2023.07.18.548845 | DOI Listing |
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