Previous research of anion channelrhodopsins (ACRs) has been performed using cytoplasmic domain (CPD)-deleted constructs and therefore have overlooked the native functions of full-length ACRs and the potential functional role(s) of the CPD. In this study, we used the recombinant expression of full-length Guillardia theta ACR1 (GtACR1_full) for pH measurements in Pichia pastoris cell suspensions as an indirect method to assess its anion transport activity and for absorption spectroscopy and flash photolysis characterization of the purified protein. The results show that the CPD, which was predicted to be intrinsically disordered and possibly phosphorylated, enhanced NO transport compared to Cl transport, which resulted in the preferential transport of NO. This correlated with the extended lifetime and large accumulation of the photocycle intermediate that is involved in the gate-open state. Considering that the depletion of a nitrogen source enhances the expression of GtACR1 in native algal cells, we suggest that NO transport could be the natural function of GtACR1_full in algal cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637977 | PMC |
| http://dx.doi.org/10.1016/j.jbc.2023.105305 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reactivity of Wet scCO toward Reservoir and Caprock Formations under Elevated Pressure and Temperature Conditions: Implications for CCS and CO-Based Geothermal Energy Extraction.
Energy Fuels
January 2025
Geothermal Energy and Geofluids Group, Institute of Geophysics, Department of Earth and Planetary Sciences, ETH Zurich, Zurich 8092, Switzerland.
Carbon capture and storage (CCS) and CO-based geothermal energy are promising technologies for reducing CO emissions and mitigating climate change. Safe implementation of these technologies requires an understanding of how CO interacts with fluids and rocks at depth, particularly under elevated pressure and temperature. While CO-bearing aqueous solutions in geological reservoirs have been extensively studied, the chemical behavior of water-bearing supercritical CO remains largely overlooked by academics and practitioners alike.
View Article and Find Full Text PDFTargeted Docking of Localized Hydrogen Bond for Efficient and Reversible Zinc-Ion Batteries.
Angew Chem Int Ed Engl
January 2025
Central South University, material science and engineering, 932 Lushan Road, 410083, Changsha, CHINA.
Hydrogen bond (HB) chemistry, a pivotal feature of aqueous zinc-ion batteries, modulates electrochemical processes through weak electrostatic interactions among water molecules. However, significant challenges persist, including sluggish desolvation kinetics and inescapable parasitic reactions at the electrolyte-electrode interface, associated with high water activity and strong Zn2+-solvent coordination. Herein, a targeted localized HB docking mechanism is activated by the polyhydroxy hexitol-based electrolyte, optimizing Zn2+ solvation structures via dipole interaction and reconstructing interfacial HB networks through preferential parallel adsorption.
View Article and Find Full Text PDF14-3-3 promotes sarcolemmal expression of cardiac Ca1.2 and nucleates isoproterenol-triggered channel superclustering.
Proc Natl Acad Sci U S A
February 2025
Department of Physiology and Membrane Biology, University of California Davis, Davis, CA 95616.
The L-type Ca channel (Ca1.2) is essential for cardiac excitation-contraction coupling. To contribute to the inward Ca flux that drives Ca-induced-Ca-release, Ca1.
View Article and Find Full Text PDFEnhanced Ion Solvation and Conductivity in Lithium-Ion Electrolytes via Tailored EMC-TMS Solvent Mixtures: A Molecular Dynamics Study.
ACS Omega
January 2025
Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
The development of stable, high-performance electrolytes is essential to addressing the safety concerns and limited lifespan caused by the thermal and chemical instability of traditional organic carbonate-based electrolytes in lithium-ion batteries (LIBs). This study examined the potential of mixed solvent systems, specifically ethyl methyl carbonate (EMC) and tetramethylene sulfone (TMS), to modify ion solvation and improve ionic conductivity in LIB electrolytes. Through molecular dynamics simulations, we investigated the solvation structure and transport properties of lithium ions (Li) in these solvent environments.
View Article and Find Full Text PDFTubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets.
Nat Commun
January 2025
Cluster of Excellence Physics of Life, TUD Dresden University of Technology, 01062, Dresden, Germany.
Cilia assembly and function rely on the bidirectional transport of components between the cell body and ciliary tip via Intraflagellar Transport (IFT) trains. Anterograde and retrograde IFT trains travel along the B- and A-tubules of microtubule doublets, respectively, ensuring smooth traffic flow. However, the mechanism underlying this segregation remains unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!