Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes. We describe steps for assembling a pupation station, self-emptying bucket, pupal funnel and dish vacuum, automatic aspirator, and sugar tubes. We also detail the application of these systems, along with specific limitations.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558827 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102621 | DOI Listing |
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