Pacific Biosciences long-read sequencing was used to improve the genome assembly for strain NRRL YB-4239 (ATCC 11503). The new assembly included eight chromosomes that were substantiated by the electrophoretic karyotype. The nuclear genome was 16.1 Mb (37.2% GC) with 5,740 genes predicted.
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| http://dx.doi.org/10.1128/MRA.00596-23 | DOI Listing |
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An improved NRRL YB-4239 genome assembly substantiated by its electrophoretic karyotype.
Microbiol Resour Announc
October 2023
Roy. J. Carver Biotechnology Center, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Pacific Biosciences long-read sequencing was used to improve the genome assembly for strain NRRL YB-4239 (ATCC 11503). The new assembly included eight chromosomes that were substantiated by the electrophoretic karyotype. The nuclear genome was 16.
View Article and Find Full Text PDFCharacterization and site-directed mutation of a novel aldo-keto reductase from Lodderomyces elongisporus NRRL YB-4239 with high production rate of ethyl (R)-4-chloro-3-hydroxybutanoate.
J Ind Microbiol Biotechnol
November 2014
College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, No. 1378, Wenyixi Road, Hangzhou, 311121, People's Republic of China.
A novel aldo-keto reductase (LEK) from Lodderomyces elongisporus NRRL YB-4239 (ATCC 11503) was discovered by genome database mining for carbonyl reduction. LEK was overexpressed in Escherichia coli BL21 (DE3), purified to homogeneity and the catalytic properties were studied. Among the substrates, ethyl 4-chloro-3-oxobutanoate was converted to ethyl (R)-4-chloro-3- hydroxybutanoate ((R)-CHBE), an important pharmaceutical intermediate, with an excellent enantiomeric excess (e.
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