Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by .

() is a contagious obligate parasite of the udder in dairy cows. Here, we examined -host interactions in bovine mammary epithelial cells (BMECs) . We found that infected BMECs through laminin β2 and integrin. Crk, Vps25, and RhoA were differentially expressed in -infected cells. infection activated FAK and Crk. FAK deficiency decreased the number of intracellular and Crk activation. Knockdown of or increased the level of intracellular , whereas its overexpression had the opposite effect. RhoA expression and actin cytoskeleton were altered in -infected BMECs. Crk and Vps25 interact in cells, and invaded also activates Crk, allowing it to cooperate with Vps25 to defend against intracellular infection by . This study provides insights into the mechanism by which intracellular infection by is regulated in BMECs.