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Single-cell transcriptomics delineates the immune cell landscape in equine lower airways and reveals upregulation of FKBP5 in horses with asthma. | LitMetric
Department of Medical Sciences, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
Published: September 2023
AI Article Synopsis
* To understand the immune response in horses with mild-to-moderate EA, researchers analyzed bronchoalveolar lavage fluid (BALF) using single-cell RNA sequencing to identify immune cell populations and their subtypes.
* The study found that FKBP5, a protein that helps regulate the glucocorticoid receptor, was significantly upregulated in horses with mild-to-moderate EA, indicating its potential role in the disease.
Article Abstract
Equine asthma (EA) is a heterogenous, complex disease, with a significant negative impact on horse welfare and performance. EA and human asthma share fundamental similarities, making EA a useful model for studying the disease. One relevant sample type for investigating chronic lung inflammation is bronchoalveolar lavage fluid (BALF), which provides a snapshot of the immune cells present in the alveolar space. To investigate the immune cell landscape of the respiratory tract in horses with mild-to-moderate equine asthma (mEA) and healthy controls, single-cell RNA sequencing was conducted on equine BALF cells. We characterized the major immune cell populations present in equine BALF, as well as subtypes thereof. Interestingly, the most significantly upregulated gene discovered in cases of mEA was FKBP5, a chaperone protein involved in regulating the activity of the glucocorticoid receptor.
