Actinoquinazolinone, a New Quinazolinone Derivative from a Marine Bacterium sp. CNQ-617, Suppresses the Motility of Gastric Cancer Cells.

A HPLC-UV guided fractionation of the culture broth of sp. CNQ-617 has led to the isolation of a new quinazolinone derivative, actinoquinazolinone (), as well as two known compounds, 7-hydroxy-6-methoxy-3,4-dihydroquinazolin-4-one () and 7-methoxy-8-hydroxy cycloanthranilylproline (). The interpretation of 1D, 2D NMR, and MS spectroscopic data revealed the planar structure of . Furthermore, compound suppressed invasion ability by inhibiting epithelial-mesenchymal transition markers (EMT) in AGS cells at a concentration of 5 µM. In addition, compound decreased the expression of seventeen genes related to human cell motility and slightly suppressed the signal transducer and activator of the transcription 3 (STAT3) signal pathway in AGS cells. Together, these results demonstrate that is a potent inhibitor of gastric cancer cells.