AI Article Synopsis
- Effective biosensing of biomolecules is crucial for detecting infectious pathogens and environmental pollutants, especially for ongoing COVID-19 challenges like SARS-CoV-2.* -
- The study focuses on improving the detection of SARS-CoV-2 nucleocapsid protein antigen using optical frequency combs (OFC), which convert antigen-antibody interaction signals into measurable radio-frequency shifts.* -
- Implementation of a dual-comb setup helps overcome temperature-related signal disturbances, enhancing the sensitivity and applicability of biosensors for various pathogens and biomarkers.*
Article Abstract
Rapid, sensitive detection of biomolecules is important for biosensing of infectious pathogens as well as biomarkers and pollutants. For example, biosensing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still strongly required for the fight against coronavirus disease 2019 (COVID-19) pandemic. Here, we aim to achieve the rapid and sensitive detection of SARS-CoV-2 nucleocapsid protein antigen by enhancing the performance of optical biosensing based on optical frequency combs (OFC). The virus-concentration-dependent optical spectrum shift produced by antigen-antibody interactions is transformed into a photonic radio-frequency (RF) shift by a frequency conversion between the optical and RF regions in the OFC, facilitating rapid and sensitive detection with well-established electrical frequency measurements. Furthermore, active-dummy temperature-drift compensation with a dual-comb configuration enables the very small change in the virus-concentration-dependent signal to be extracted from the large, variable background signal caused by temperature disturbance. The achieved performance of dual-comb biosensing will greatly enhance the applicability of biosensors to viruses, biomarkers, environmental hormones, and so on.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522648 | PMC |
| http://dx.doi.org/10.1038/s41598-023-41436-3 | DOI Listing |
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