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Use of next-generation sequencing to detect mutations associated with antiviral drug resistance in cytomegalovirus. | LitMetric

AI Article Synopsis

  • Cytomegalovirus (CMV) poses serious health risks for immunocompromised patients, such as those who have had organ transplants, making antiviral treatment essential to prevent disease.
  • Traditional testing for antiviral resistance in CMV relied on Sanger sequencing, but a new next-generation sequencing (NGS) method was developed for better detection of mutations related to resistance.
  • This study found that the NGS method matched Sanger sequencing in identifying resistance 100% of the time, and can help healthcare providers customize antiviral therapies for CMV-infected patients with resistance mutations.

Article Abstract

Cytomegalovirus (CMV) is a significant cause of morbidity and mortality among immunocompromised hosts, including transplant recipients. Antiviral prophylaxis or treatment is used to reduce the incidence of CMV disease in this patient population; however, there is concern about increasing antiviral resistance. Detection of antiviral resistance in CMV was traditionally accomplished using Sanger sequencing of and genes, in which specific mutations may result in reduced antiviral activity. In this study, a novel next-generation sequencing (NGS) method was developed and validated to detect mutations in / associated with antiviral resistance. Plasma samples ( = 27) submitted for antiviral resistance testing by Sanger sequencing were also analyzed using the NGS method. When compared to Sanger sequencing, the NGS assay demonstrated 100% (27/27) overall agreement for determining antiviral resistance/susceptibility and 88% (22/25) agreement at the level of resistance-associated mutations. The limit of detection of the NGS method was determined to be 500 IU/mL, and the lower threshold for detecting mutations associated with resistance was established at 15%. The NGS assay represents a novel laboratory tool that assists healthcare providers in treating patients who are infected with CMV harboring resistance-associated mutations and who may benefit from tailored antiviral therapy.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10595055PMC
http://dx.doi.org/10.1128/jcm.00429-23DOI Listing

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