The Cxcr2 subset of the S100a8 gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathology.

Introduction: Gastric myeloid-derived suppressor cells (MDSCs) are a prominent population that expands during gastric pre-neoplastic and neoplastic development in humans and mice. However, the heterogeneity of this population has circumvented the ability to study these cells or understand their functions. Aside from Schlafen-4 (Slfn-4) MDSCs in mouse studies, which constitute a subset of this population, limitations exist in characterizing the heterogeneity of the gastric CD11bLy6G population and targeting its different subsets. Here we identify S100a8 as a pan-specific marker for this population and utilize it to study the role of the S100a8Cxcr2 subset.

Methods: We profiled gastric CD11bLy6G versus CD11bLy6G myeloid cells by transcriptomic and single-cell RNA sequencing. We identified S100a8 as a pan-specific marker of the gastric granulocytic MDSC (G-MDSC) population, and generated S100a8Cxcr2 to study the effects of Cxcr2 knockdown.

Results: Following 6-months of infection, gastric CD11bLy6G G-MDSCs were highly enriched for the expression of S100a8, S100a9, Slfn4, Cxcr2, Irg1, Il1f9, Hcar2, Retnlg, Wfdc21, Trem1, Csf3R, Nlrp3, and Il1b. The expression of these distinct genes following 6mo infection marked heterogeneous subpopulations, but they all represented a subset of S100a8 cells. S100a8 was identified as a pan-marker for CD11bLy6G cells arising in chronic inflammation, but not neutrophils recruited during acute gut infection. 6mo -infected S100a8Cxcr2 mice exhibited worsened gastric metaplastic pathology than Cxcr2 mice, which was associated with dysregulated lipid metabolism and peroxidation.

Conclusion: S100a8 is a pan-specific marker that can be used to target gastric G-MDSC subpopulations, of which the Cxcr2 subset regulates gastric immunopathology and associates with the regulation of lipid peroxidation.