Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from , a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth. CoCas9 recognizes a novel 5'-NRRWC-3' PAM and efficiently cleaves DNA . Functional characterization of CoCas9 opens ways for genetic engineering of using its endogenous CRISPR-Cas system. The novel PAM requirement makes CoCas9 potentially useful in genome editing applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10521337 | PMC |
| http://dx.doi.org/10.1080/15476286.2023.2256578 | DOI Listing |
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Characterization of CoCas9 nuclease from .
RNA Biol
January 2023
Center of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia.
Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from , a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth.
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