ATP-triggered, selective superoxide radical generating oxidase-mimetic cerium oxide nanozyme exhibiting efficient antibacterial activity at physiological pH.

Colloids Surf B Biointerfaces

DBT-National Institute of Animal Biotechnology (NIAB), Opposite Journalist Colony, Near Gowlidoddy, Extended Q-City Road, Gachibowli, Hyderabad 500032, Telangana, India; DBT-Regional Centre for Biotechnology (RCB), Faridabad 121001, Haryana, India. Electronic address:

Published: November 2023

Bacterial infections are considered as one of the major health threats to the global population. The advent of bacterial species with antibiotic resistance has attracted significant efforts to develop novel materials and strategies to effectively avoid the resistance with enhanced antibacterial potential. In this work, we have developed oxidase-mimetic cerium oxide nanoparticles (CeO NPs), which exhibit nanozyme activity at physiological pH in the presence of adenosine triphosphate (ATP). The oxidase-mimetic activity was confirmed to involve superoxide radicals using p-benzoquinone and dihydroethidium. Using indole propionic acid, ethanol, and terephthalic acid, it was confirmed that the oxidase-mimetic activity of CeO NPs with ATP does not involve the formation of hydroxyl radicals. CeO NPs with ATP produced a strong antibacterial activity against Staphylococcus aureus and Escherichia coli within 3 - 6 hrs. The bacterial cell morphology analysis suggested that superoxide radicals generated during the oxidase-mimetic activity of CeO NPs with ATP cause distortion of paired and tetrad arrangement (Staphylococcus aureus), loss of cytoplasmic content, damage, and pore formation in the cell wall (Escherichia coli) that led to the death of bacteria. Further, the live/dead assay also concludes the time-dependent death of bacterial cells with the highest death in the cell population exposed to CeO NPs and ATP. Thus, the antibacterial activity at physiological pH by superoxide radical generating oxidase-mimetic CeO NPs could be further extended to other pathogenic bacterial species.

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Source
http://dx.doi.org/10.1016/j.colsurfb.2023.113531DOI Listing

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