Isolation, screening and characterization of phage.

Prog Mol Biol Transl Sci

Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza, Egypt; Faculty of Environmental Agricultural Sciences, Arish University, Arish, Egypt.

Published: September 2023

Bacterial resistance threatens public health due to a lack of novel antibacterial classes since the 21 century. Bacteriophages, the most ubiquitous microorganism on Earth and natural predators of bacteria, have the potential to save the world from the post-antibiotic era. Therefore, phage isolation and characterization are in high demand to find suitable phages for therapeutic and bacterial control applications. The chapter presents brief guidance supported by recommendations on the isolation of phages, and initial screening of phage antimicrobial efficacy, in addition to, conducting comprehensive characterization addressing morphological, biological, genomic, and taxonomic features.

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Source
http://dx.doi.org/10.1016/bs.pmbts.2023.03.008DOI Listing

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