Food allergy is a current worldwide problem. Consequently, it is necessary to identify the different molecules that modulate these reactions and that can be used as potential biomarkers. In recent years there has been increasing interest in the field of allergy on microRNAs (miRNAs). These molecules regulate a wide variety of physiological processes and have been proposed as promising candidate biomarkers.Currently, next-generation sequencing (NGS) has allowed to determine the profile of all miRNAs from different samples. In addition, there are several methods to extract RNA and miRNAs, from different sources such as serum, extracellular vesicles (EVs), and/or cell extracts. Following extraction, a retrotranscription step must be carried out before miRNA levels can be quantified by quantitative polymerase chain reaction (qPCR).This chapter aimed to describe the discovery techniques used to determine the differential profile of miRNAs from different types of samples, as well as the diverse methods employed to extract these molecules and quantify specific changes in their levels by qPCR.
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Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class.
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January 2025
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:
The membrane-proximal external region (MPER) of the HIV-1 envelope is a target for broadly neutralizing antibodies (bnAbs), and vaccine-elicited MPER-directed antibodies have recently been reported from a human clinical trial. In this study, we sought to identify MPER-directed nAbs in simian immunodeficiency virus (SIV)-infected rhesus macaques. We isolated four lineages of SIV MPER-directed nAbs from two SIV-infected macaques.
View Article and Find Full Text PDFThe baked side: Cow's milk and egg protein threshold dose distributions in children reacting to baked milk and baked egg.
World Allergy Organ J
January 2025
Allergy Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Background: Children allergic to milk and egg, but tolerant to baked products, display higher reactivity thresholds than the general population of children allergic to milk and egg. We sought to verify the reactivity thresholds of milk- and egg-allergic children who also react to baked milk and baked egg, respectively.
Methods: We retrospectively assessed consecutive oral food challenge (OFC) for baked milk and egg between January 2018 and March 2022 in a population of baked milk- and baked-egg allergic children.
IgE-based analysis of sensitization and cross-reactivity to yellow mealworm and edible insect allergens before their widespread dietary introduction.
Sci Rep
January 2025
Department of Experimental Allergology and Immunology, Medical University of Bialystok, Bialystok, Poland.
The European Commission authorized the use of dried yellow mealworm (Tenebrio molitor - TM) as a food ingredient under Regulation EU 2021/882. As TM emerges as an important allergen source, sensitization and allergy to TM in various populations need investigation. The aim of this study was to assess the incidence of sensitization to TM before its introduction as a food ingredient in Poland, as well as checking the occurrence of co-sensitivity to TM and other invertebrate allergenic extracts and molecules.
View Article and Find Full Text PDFAnaphylaxis in infancy: An area that needs to be highlighted.
Allergol Immunopathol (Madr)
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Department of Pediatrics, University Hospital of Ioannina, Ioannina, Greece.
Anaphylaxis, the most severe end of the spectrum of allergic reactions, has shown increasing incidence globally over recent years. This hypersensitivity reaction can occur at any age, including infancy. Recent data, although scarce, show that anaphylaxis is increasingly reported in infancy, with food identified as the leading cause of anaphylaxis cases in this age group.
View Article and Find Full Text PDFThe effect of formula type on the prognosis of allergic proctocolitis due to cow's milk allergy.
Allergol Immunopathol (Madr)
January 2025
Department of Pediatric Allergy and Immunology, Cemil Tascioglu City Hospital, University of Health Sciences, Istanbul, Turkey.
Background: Food protein-induced allergic proctocolitis is a nonimmunoglobulin E-mediated, self-limited food allergy of the rectum and the colon. Cow's milk protein is the most common allergen responsible for the disease.
Objective: This study aimed to investigate the roles of different types of formulas in building early tolerance to food protein-induced allergic proctocolitis in infants.
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