First Report of Antibiotic Resistance Markers A and Among Group Strains in Ecuadorian Patients.

In recent years, increasing resistance of to several antibiotics has been reported in different countries. The aim of this study was to evaluate the antibiotic resistance profiles of spp. isolated from clinical samples by phenotypic and molecular methods. A total of 40 nonrepetitive isolates of the group were studied from 2018 to 2019. The species was identified by API 20A system. The minimum inhibitory concentrations (MICs) were determined by Sensititre anaerobe MIC plate. The presence of the and genes was checked by conventional PCR. The association between genes and insertion sequence (IS) was performed by whole genome sequencing. Eleven isolates were categorized as metronidazole-resistant and only 2 isolates harbored the gene. Five isolates were imipenem-resistant, but gene was detected in two isolates. A gene was closely related to the A-4 allele and associated with IS614B. The gene was not related to any gene type and was considered a new variant named L. IS612 was found upstream of L gene. In view of the scarcity of data on , there is a need to surveil antibiotic resistance levels and molecular mechanisms to implement better antimicrobial therapies against this important group of bacteria.