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Neutral lipids restrict the mobility of broken DNA molecules during comet assays.
Biol Cell
January 2025
Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier, Centre National de la Recherche Scientifique, Montpellier, France.
One widespread technique to assess in relative terms the amount of broken DNA present in the genome of individual cells consists of immobilizing the cell's nucleus under an agarose pad (called the nucleoid) and subjecting the whole genome to electrophoresis to force broken DNA molecules out of it. Since the migrating broken DNA molecules create a tail behind the nucleoid, this technique is named the comet assay. While performing comet assays regularly, we systematically observed circular regions devoid of DNA within the nucleoid region.
View Article and Find Full Text PDFThe anterior offset of the standard entry point for tibial intramedullary nails: A transparent 3D-CT image based analysis.
J Orthop
August 2025
Department of Orthopaedic Surgery, Oita University Hospital, Japan.
Background: This study examines the relationship between the anterior offset of the tibial intramedullary nail (TIN) entry point and the tibial shaft axis to enhance the fit and alignment of TINs, using transparent 3D-CT in an accurate lateral view to minimize rotational artifacts.
Methods: Data were collected from 100 adult patients undergoing tibial CT scans. Measurements included the anterior offset from the tibial axis to the entry point, tibial tubercle offset, tibial plateau length, posterior slope, tibial length, isthmus diameter, and the isthmus-to-tibial length ratio.
Comprehensive evaluation of the toxicological effects of commonly encountered synthetic cathinones using in silico methods.
Toxicol Res (Camb)
February 2025
Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy.
Synthetic cathinones (SCs), a group of new psychoactive substances (NPS), are designer molecules with hallucinogenic and psychostimulatory effects. Although the structural similarities of SCs to amphetamines suggest that they may have similar toxicity profiles to those of amphetamine congeners, little is known about SCs from a toxicological point of view. In the present study, the toxicity profiles of commonly encountered SCs ( = 65), listed in the 2020 Report of the United Nations Office on Drugs and Crime (UNODC), were evaluated using in silico methods.
View Article and Find Full Text PDFA qualitative study to explain the determinants of naswār consumption from the point of view of its consumers.
J Educ Health Promot
December 2024
Department of Nursing School, Gerash University of Medical Sciences, Gerash, Iran.
Background: One of the health problems related to tobacco that is increasing in society is the consumption of naswār. This study aimed to investigate the determinants of naswār consumption from the point of view of its consumers.
Materials And Methods: In this qualitative study, 47 men with an age range of 23- 54 years who consumed naswār from the southern cities of Fars province in Iran were selected and interviewed.
Comparative electron diffraction analysis of strain relaxation in AlxGa1-xN materials in the microelectronics industry: 4D-STEM approach vs. TEM-based N-PED solution.
Micron
January 2025
CEMES-CNRS, 29 Rue Jeanne Marvig, Toulouse 31055, France.
Owing to its high spatial resolution and its high sensitivity to chemical element detection, transmission electron microscopy (TEM) technique enables to address high-level materials characterization of advanced technologies in the microelectronics field. TEM instruments fitted with various techniques are well-suited for assessing the local structural and chemical order of specific details. Among these techniques, 4D-STEM is suitable to estimate the strain distribution of a large field of view.
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