AI Article Synopsis
- In certain species, one oocyte is developed from a group of interconnected germline sister cells, while the others act as nurse cells that support the oocyte by transporting necessary materials.
- The microtubule polymerase Mini spindles (Msps) is crucial for maintaining the oocyte's specification by transporting mRNA to the oocyte and promoting microtubule growth.
- A positive feedback loop is created by Msps and dynein, which boosts microtubule activity in the oocyte and enhances the transport of materials from nurse cells, ensuring the oocyte's development and stability.
Article Abstract
In many species, only one oocyte is specified among a group of interconnected germline sister cells. In , 16 interconnected cells form a germline cyst, where one cell differentiates into an oocyte, while the rest become nurse cells that supply the oocyte with mRNAs, proteins, and organelles through intercellular cytoplasmic bridges named ring canals via microtubule-based transport. In this study, we find that a microtubule polymerase Mini spindles (Msps), the homolog of XMAP215, is essential for maintenance of the oocyte specification. mRNA encoding Msps is transported and concentrated in the oocyte by dynein-dependent transport along microtubules. Translated Msps stimulates microtubule polymerization in the oocyte, causing more microtubule plus ends to grow from the oocyte through the ring canals into nurse cells, further enhancing nurse cell-to-oocyte transport by dynein. Knockdown of blocks the oocyte growth and causes gradual loss of oocyte determinants. Thus, the Msps-dynein duo creates a positive feedback loop, ensuring oocyte fate maintenance by promoting high microtubule polymerization activity in the oocyte, and enhancing dynein-dependent nurse cell-to-oocyte transport.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523470 | PMC |
| http://dx.doi.org/10.1073/pnas.2303376120 | DOI Listing |
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