Temperature-sensitive (TS) alleles create tunable thermoswitches to deplete essential cellular activities and are used to dissect gene function. In their recent study, Link and colleagues (Schramm et al 2023) use a CRISPR-based approach to systematically create TS alleles across essential genes in E. coli.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568200 | PMC |
| http://dx.doi.org/10.15252/msb.202311933 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Extreme heat risk and the potential implications for the scheduling of football matches at the 2026 FIFA World Cup.
Int J Biometeorol
January 2025
Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, ON, Canada.
Climate change is making extreme heat events more frequent and intense. This negatively impacts many aspects of society, including organised sport. As the world's most watched sporting event, the FIFA World Cup commands particular attention around the threat of extreme heat.
View Article and Find Full Text PDFA method of coating life prediction based on high temperature thermal shock life test and three-dimensional heat transfer analysis.
Sci Rep
January 2025
School of Instrumental Science and Engineering, Harbin Institute of Technology, 150001, Harbin, People's Republic of China.
In this study, a method for predicting the thermal shock life of coatings is proposed, and a model for predicting the thermal shock life of coatings based on high temperature thermal shock life test and three-dimensional heat transfer analysis is established. Firstly, the thermal shock life of coatings at different cooling and heating cycle temperatures is obtained through a designed thermal shock life testing device for silicide coatings at a wide-temperature range from 500℃ to 3000℃. Secondly, the actual thickness of the coating and the continuous oxidation in the thermal shock life test are taken into consideration.
View Article and Find Full Text PDFLong-term safety of lentiviral or gammaretroviral gene-modified T cell therapies.
Nat Med
January 2025
Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Long-term risks of gene therapy are not fully understood. In this study, we evaluated safety outcomes in 783 patients over more than 2,200 total patient-years of observation from 38 T cell therapy trials. The trials employed integrating gammaretroviral or lentiviral vectors to deliver engineered receptors to target HIV-1 infection or cancer.
View Article and Find Full Text PDFIn vitro evaluation of the immunomodulatory and antibacterial activities of calcitriol (1,25-dihydroxy-vitamin D3) as a potential application for aerobic vaginitis treatment - preliminary study results.
BMC Microbiol
January 2025
Chair of Microbiology, Jagiellonian University Medical College in Krakow, 18 Czysta Street, Cracow, 31-121, Poland.
Background: Aerobic vaginitis (AV) is a state of abnormal vaginal microbiota, which is associated with increased numbers of aerobic, enteric bacteria and inflammation of the vaginal epithelium. Anti-microbial treatment combined with anti-inflammatory therapy could be useful in the treatment of this condition. It is known that calcitriol, the active form of vitamin D, plays an important role in modulating the immune response in several inflammatory diseases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!