Biodegradation of 1,4-dioxane in a continuous-flow bioelectrochemical reactor by biofilm of Pseudonocardia dioxanivorans CB1190 and microbial community on conductive carriers.

Bioelectrochemical degradation is an environmentally friendly, cost-effective and controllable way of providing electron acceptor to the microorganisms. A two-chamber continuous-flow bioelectrochemical reactor (BER) was developed in this study. The objective was to investigate the potential for enhancing the bioelectrochemical degradation of 1,4-dioxane (DX) by Pseudonocardia dioxanivorans CB1190 (CB1190) and microbial community biofilm on conductive and non-conductive carriers in low potentials (1.0-1.2 V) and currents (<2 mA). In the case of CB1190, biodegradation experiments at 1.0 V did not result in any observable change in DX removal efficiency (32.63 ± 2.48%) compared to the 0.0 V (31.69 ± 2.33%). However, the removal efficiency was much higher at 1.2 V (59.08 ± 0.86%). The higher removal at 1.2 V was attributed to an increase in dissolved oxygen (DO) concentration from 3.77 ± 0.33 mg/L at 0.0 V to 5.40 ± 0.11 mg/L at 1.2 V, which resulted from water electrolysis. In the case of microbial community, on the other hand, DX removal efficiency increased at 1.0 V (30.98 ± 1.10%) compared to 0.0 V (23.40 ± 1.02%) that can be attributed to a simultaneous increase in microbial activity from 2389 ± 118.5 ngATP/mgVSS at 0.0 V to 2942 ± 109 ngATP/mgVSS at 1.0 V. Analysis of the changes in microbial composition indicated enrichment of Alistipes and Lutispora at 1.0 V due to the ability of these genera to directly transfer electrons with conductive surface. On the other hand, no change was observed in the microbial community in the case of non-conductive carriers. Results of this study showed that electro-assisted biodegradation of DX at low potentials is possible through two different mechanisms (oxygen production and direct electron transfer with electrode) which makes this technique flexible and cost-effective. The novelty of this work lies in exploring the use of electrical assistance to enhance the biodegradation of DX in the presence of CB1190 and the microbial community. This study more specifically investigated lower potential than required water electrolysis potential, allowing microorganisms to be stimulated through mechanisms unrelated to oxygen generation.