The cortical organoid is an efficient model for studying human brain neurodevelopment and neurological disease. However, its three-dimensional structure limits real-time observation of internal physiological changes. Here, we present a protocol for an air-liquid interface attachment culture for cortical organoids. We describe steps for transplanting cortical organoid slices and generating the air-liquid interface. We then detail calcium imaging on organoid external neural networks and immunohistochemical staining on confocal plates.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502426 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102502 | DOI Listing |
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