One of the chitinases (ChiG) derived from the chitinolytic bacterium Chitiniphilus shinanonensis SAY3T exhibited chitobiase activity cleaving dimers of N-acetyl-D-glucosamine (GlcNAc) into monomers, which is not detected in typical endo-type chitinases. Analysis of the reaction products for GlcNAc hexamers revealed that all the five internal glycosidic bonds were cleaved at the initial stage. The overall reaction catalyzed by chitobiases toward GlcNAc dimers was similar to that catalyzed by N-acetyl-D-glucosaminidases (NAGs). SAY3 possesses two NAGs (ChiI and ChiT) that are thought to be important in chitin catabolism. Unexpectedly, a triple gene-disrupted mutant (ΔchiIΔchiTΔchiG) was still able to grow on synthetic medium containing GlcNAc dimers or powdered chitin, similar to the wild-type SAY3, although it exhibited only 3% of total cellular NAG activity compared to the wild-type. This indicates the presence of unidentified enzyme(s) capable of supporting normal bacterial growth on the chitin medium by NAG activity compensation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/bbb/zbad134 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Altitude's Impact on the Rhizosphere Prokaryotic Communities of the Cretan Endemic Plant (L.) A.DC.
Microorganisms
January 2025
Department of Ecology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
The present study examined the effect of the three different altitudes on the enzymatic activity and the prokaryotic communities of the rhizosphere of (L.) A.DC.
View Article and Find Full Text PDFComposition of soil fungal communities and microbial activity along an elevational gradient in Mt. Jiri, Republic of Korea.
PeerJ
January 2025
Department of Environmental Engineering, Konkuk University, Seoul, Republic of Korea.
Approximately 64% of the Republic of Korea comprises mountainous areas, which as cold and high-altitude regions are gravely affected by climate change. Within the mountainous and the alpine-subalpine ecosystems, microbial communities play a pivotal role in biogeochemical cycling and partly regulate climate change through such cycles. We investigated the composition and function of microbial communities, with a focus on fungal communities, in Republic of Korea's second tallest mountain, Mt.
View Article and Find Full Text PDFEffects of a β-glucan-enriched diet on biomarkers of oxidative stress, energy metabolism and lysosomal function in muscle tissue of European grayling ( L.).
J Vet Res
December 2024
Institute of Biology, Pomeranian University in Słupsk, 76-200 Słupsk, Poland.
Introduction: The grayling ( L.) has several advantages over other fish species that make it attractive for aquaculture and invest it with importance for food security. The study assessed the effects of a β-glucan-enriched diet on biomarkers of oxidative stress, energy metabolism and lysosomal function in muscle tissue of European grayling ( L.
View Article and Find Full Text PDFA Drosophila Model of Mucopolysaccharidosis IIIB.
Genetics
December 2024
Department of Genetics and Biochemistry and Center for Human Genetics, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC 29646, USA.
Article Synopsis
- MPS IIIB is a rare disorder caused by defects in the enzyme NAGLU, leading to brain dysfunction due to the accumulation of heparan sulfate in lysosomes.
- Researchers created a Drosophila (fruit fly) model with various NAGLU mutations to study the disorder's effects on activity and sleep patterns, revealing significant hyperactivity and sleep issues.
- The study found that gene expression changes in mutant flies are linked to problems with nervous system development and synaptic function, suggesting that this fly model could help develop future therapies for MPS IIIB.
Nitrogen Deposition Weakens Soil Carbon Control of Nitrogen Dynamics Across the Contiguous United States.
Glob Chang Biol
December 2024
Stockbridge School of Agriculture, University of Massachusetts Amherst, Amherst, Massachusetts, USA.
Anthropogenic nitrogen (N) deposition is unequally distributed across space and time, with inputs to terrestrial ecosystems impacted by industry regulations and variations in human activity. Soil carbon (C) content normally controls the fraction of mineralized N that is nitrified (ƒ), affecting N bioavailability for plants and microbes. However, it is unknown whether N deposition has modified the relationships among soil C, net N mineralization, and net nitrification.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!