Effect of insulin on IR and GLP1-R expressions in HT22 cells.

Med Oncol

Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.

Published: September 2023

Insulin is a significant growth factor that specifically binds to the insulin receptor (IR) in the brain and then activates the PI3K-AKT pathway. Glucagon-like peptide 1 (GLP-1) has a variety of functions including neuroprotection, support for neurogenesis, and increasing insulin signal. This study aims to investigate the effect of insulin administered to immortalized clonal mouse hippocampal cell line (HT22) at different doses and intervals on IR, insulin receptor A (IRA), insulin receptor B (IRB), and Glucagon-like peptide 1 receptor (GLP1-R) mRNA expression and protein levels. The cells were planted in 6 well plates at a density of 3 × 10/4 × 10. Cells treated with insulin at different concentrations (5, 10, and 40 nM) were collected at 0.5, 2, 8, 16, and 24 h. RT-PCR and western blot analysis were used to measure mRNA expression and protein levels. Our results showed that insulin has short and long-term effects on IR and GLP1-R expression depending on dose and time. These findings may guide future studies targeting IR isoforms and GLP1-R in particular, as well as determining the optimal dose and duration of insulin stimulation in insulin signaling research.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12032-023-02172-wDOI Listing

Publication Analysis

Top Keywords

insulin receptor
12
insulin
11
glucagon-like peptide
8
mrna expression
8
expression protein
8
protein levels
8
insulin glp1-r
4
glp1-r expressions
4
expressions ht22
4
ht22 cells
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!