A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

NaCl-induced modulation of species distribution in a mixed / / biofilm. | LitMetric

NaCl-induced modulation of species distribution in a mixed / / biofilm.

Biofilm

Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France.

Published: December 2023

AI Article Synopsis

  • Cystic fibrosis (CF) patients' lungs provide a unique environment with high NaCl concentrations that promote the growth of osmotolerant pathogens and weaken the body's antibacterial defenses, leading to increased infection risk.
  • The study investigates how elevated NaCl levels (145 mM) affect the interactions and biofilm formation of multiple bacterial species, including those that are clinically relevant, using various experimental techniques.
  • Findings reveal that high NaCl concentrations not only enhance biofilm growth but also foster complex bacterial relationships, suggesting a significant role of the microenvironment in the behavior and development of multi-species biofilms in CF patients.

Article Abstract

, , and are notorious pathogens known for their ability to form resilient biofilms, particularly within the lung environment of cystic fibrosis (CF) patients. The heightened concentration of NaCl, prevalent in the airway liquid of CF patients' lungs, has been identified as a factor that promotes the growth of osmotolerant bacteria like and dampens host antibacterial defenses, thereby fostering favorable conditions for infections. In this study, we aimed to investigate how increased NaCl concentrations impact the development of multi-species biofilms , using both laboratory strains and clinical isolates of , , and B. cepacia co-cultures. Employing a low-nutrient culture medium that fosters biofilm growth of the selected species, we quantified biofilm formation through a combination of adherent CFU counts, qPCR analysis, and confocal microscopy observations. Our findings reaffirmed the challenges faced by in establishing growth within 1:1 mixed biofilms with when cultivated in a minimal medium. Intriguingly, at an elevated NaCl concentration of 145 mM, a symbiotic relationship emerged between and , enabling their co-existence. Notably, this hyperosmotic environment also exerted an influence on the interplay of these two bacteria with . We demonstrated that elevated NaCl concentrations play a pivotal role in orchestrating the distribution of these three species within the biofilm matrix. Furthermore, our study unveiled the beneficial impact of NaCl on the biofilm growth of clinically relevant mucoid strains, as well as two strains of methicillin-sensitive and methicillin-resistant . This underscores the crucial role of the microenvironment during the colonization and infection processes. The results suggest that hyperosmotic conditions could hold the key to unlocking a deeper understanding of the genesis and behavior of CF multi-species biofilms.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497989PMC
http://dx.doi.org/10.1016/j.bioflm.2023.100153DOI Listing

Publication Analysis

Top Keywords

nacl concentrations
8
multi-species biofilms
8
biofilm growth
8
elevated nacl
8
biofilm
5
nacl
5
nacl-induced modulation
4
modulation species
4
species distribution
4
distribution mixed
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!