AI Article Synopsis
- The text explores how doughnut beam illumination in image scanning microscopy enhances signal strength and integrated intensity.
- It highlights that this method provides improved optical sectioning and reduces background interference compared to traditional Airy disk illumination.
- Additionally, it mentions that digital processing of signals from outer detector pixels can further enhance these effects through techniques like subtraction.
Article Abstract
We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumination. The outer pixels of a detector array give a signal from defocused regions, so digital processing of these (e.g., by simple subtraction) can further improve optical sectioning and background rejection from a single in-focus scan.
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| http://dx.doi.org/10.1364/JOSAA.495984 | DOI Listing |
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