Isolation and identification of endophytic actinobacteria from and plants and investigation of their effects on germination and growth of wheat plant.

Plant biotechnology helps to develop different types of new products with increased resistance to disease, greater tolerance to drought and salt stress, and better nutritional value. The interaction of plants and microorganisms will play a significant role to achieve this purpose. The aims of this study were to isolate endophyte strains of some medicinal plants and the investigation of their bioactive potential. 15 strains were selectively isolated from and plants, and then their belonging to phylum was confirmed using an -specific primer pair. The antioxidant activity of the crude extract obtained from the isolated strains was investigated based on DPPH method. Investigating the antioxidant activity of the crude extract showed that at a concentration of 100 μg/mL, the two strains EG1 and EG2 had 71% and 78% antioxidant activity, respectively. According to the phylogeny studies, it was determined that two strains belonged to the genus. The effect of supernatant achieved from selected endophytic strain on 35-day wheat plants showed that the supernatant considerably promotes root and shoot growth and chlorophyll content under salinity stress (150 mM NaCl). In general, it can be concluded strains that live symbiotically with medicinal plants are rich sources of bioactive compounds. Therefore, identification of the bioactive compounds in the extract of isolated from medicinal plants and further studies on their metabolism are suggested.