AI Article Synopsis

  • * Research on Corynebacterium glutamicum reveals that resveratrol accumulates in the cell membrane and wall, which leads to membrane rigidity and affects cell viability.
  • * Supplementing with certain fatty acids, specifically palmitelaidic and linoleic acid, can reduce the toxic effects of resveratrol, resulting in a three-fold increase in production, suggesting this strategy could be applied to other bioproducts as well.

Article Abstract

Microbial synthesis of nutraceutically and pharmaceutically interesting plant polyphenols represents a more environmentally friendly alternative to chemical synthesis or plant extraction. However, most polyphenols are cytotoxic for microorganisms as they are believed to negatively affect cell integrity and transport processes. To increase the production performance of engineered cell factories, strategies have to be developed to mitigate these detrimental effects. Here, we examine the accumulation of the stilbenoid resveratrol in the cell membrane and cell wall during its production using Corynebacterium glutamicum and uncover the membrane rigidifying effect of this stilbenoid experimentally and with molecular dynamics simulations. A screen of free fatty acid supplements identifies palmitelaidic acid and linoleic acid as suitable additives to attenuate resveratrol's cytotoxic effects resulting in a three-fold higher product titer. This cost-effective approach to counteract membrane-damaging effects of product accumulation is transferable to the microbial production of other polyphenols and may represent an engineering target for other membrane-active bioproducts.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497605PMC
http://dx.doi.org/10.1038/s41467-023-40947-xDOI Listing

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