In order to express the quantitative value by the cytochemical method, the breast cancer cells of 41 human and 7 C3H mice were used. The condition of ER was detected by cytochemical (17-fluoresceinated estrone, 17-FE) and biochemical (dextran-coated charcoal, D. C. C.) methods. The relative weight (theta) of each type of cells and the coefficient were obtained by the statistical estimation technique and mathematical modeling. The relative weights of type A, B and C (theta 1, theta 2 and theta 3) were 4.4, 2.8 and 1.8 in human tumor, and 1.9, 0.3 and 1.3 in mouse tumor. The same quantitative unit of value (fmol/mg) can be expressed by the cytochemical method as by the biochemical method. The inadequacies of both methods are presently brought to light. By the D. C. C. method, only about half of the ER in cytosol can be detected and those in the nucleus can not be assayed. By the 17-FE method, it is possible to detect the ER status not only morphologically but also quantitatively.
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