AI Article Synopsis
- Nitroaromatic compounds are useful in industry but their production is often dangerous and not very efficient.
- Researchers developed a genetically modified E. coli strain called PN-1 that successfully produces p-nitrobenzoate (PNBA) from glucose by disrupting genes involved in PNBA degradation and expressing a specific enzyme.
- Further enhancements led to a new strain, PN-4Ap, which produced higher amounts of PNBA (975 mg/L) by disrupting genes that compete for resources, showcasing the ability of microbes for sustainable nitroaromatic compound production.
Article Abstract
Nitroaromatic compounds are widely used in industry, but their production is associated with issues such as the hazardousness of the process and low regioselectivity. Here, we successfully demonstrated the production of p-nitrobenzoate (PNBA) from glucose by constructing p-aminobenzoate N-oxygenase AurF-expressing E. coli. We generated this strain, which we named PN-1 by disrupting four genes involved in PNBA degradation: nfsA, nfsB, nemA, and azoR. We then expressed AurF from Streptomyces thioluteus in this strain, which resulted in the production of 945 mg/L PNBA in the presence of 1 g/L p-aminobenzoate. Direct production of PNBA from glucose was achieved by co-expressing the pabA, pabB, and pabC, as well as aurF, resulting in the production of 393 mg/L PNBA from 20 g/L glucose. To improve the PNBA titer, we disrupted genes involved in competing pathways: pheA, tyrA, trpE, pykA, and pykF. The resultant strain PN-4Ap produced 975 mg/L PNBA after 72 h of cultivation. These results highlight the potential of using microorganisms to produce other nitroaromatic compounds.
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| http://dx.doi.org/10.1016/j.enzmictec.2023.110321 | DOI Listing |
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Article Synopsis
- Nitroaromatic compounds are useful in industry but their production is often dangerous and not very efficient.
- Researchers developed a genetically modified E. coli strain called PN-1 that successfully produces p-nitrobenzoate (PNBA) from glucose by disrupting genes involved in PNBA degradation and expressing a specific enzyme.
- Further enhancements led to a new strain, PN-4Ap, which produced higher amounts of PNBA (975 mg/L) by disrupting genes that compete for resources, showcasing the ability of microbes for sustainable nitroaromatic compound production.
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