The mechanism of regulation of natamycin biosynthesis by Streptomyces in response to oxidative stress is unclear. Here, we first show cholesterol oxidase SgnE, which catalyzes the formation of HO from sterols, triggered a series of redox-dependent interactions to stimulate natamycin production in . In response to reactive oxygen species, residues Cys212 and Cys221 of the HO-sensing consensus sequence of OxyR were oxidized, resulting in conformational changes in the protein: OxyR extended its DNA-binding domain to interact with four motifs of promoter p . This acted as a redox-dependent switch to turn on/off gene transcription of , which encodes a cluster-situated regulator, by controlling the affinity between OxyR and p , thus regulating the expression of 12 genes in the natamycin biosynthesis gene cluster. OxyR cooperates with SgnR, another cluster-situated regulator and an upstream regulatory factor of SgnM, synergistically modulated natamycin biosynthesis by masking/unmasking the -35 region of p depending on the redox state of OxyR in response to the intracellular HO concentration. IMPORTANCE Cholesterol oxidase SgnE is an indispensable factor, with an unclear mechanism, for natamycin biosynthesis in . Oxidative stress has been attributed to the natamycin biosynthesis. Here, we show that SgnE catalyzes the formation of HO from sterols and triggers a series of redox-dependent interactions to stimulate natamycin production in . OxyR, which cooperates with SgnR, acted as a redox-dependent switch to turn on/off gene transcription of , which encodes a cluster-situated regulator, by masking/unmasking its -35 region, to control the natamycin biosynthesis gene cluster. This work provides a novel perspective on the crosstalk between intracellular ROS homeostasis and natamycin biosynthesis. Application of these findings will improve antibiotic yields via control of the intracellular redox pressure in .
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http://dx.doi.org/10.1128/spectrum.00879-23 | DOI Listing |
ACS Appl Mater Interfaces
November 2024
Department of Ophthalmology, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong Province 266003, China.
Food Sci Biotechnol
November 2024
College of Food and Bioengineering, Henan University of Science & Technology, Luoyang, 471023 China.
Unlabelled: L-valine (L-Val) was previously confirmed to promote natamycin biosynthesis in HW-2. In this study, natamycin yield was 1.9-fold increase with 0.
View Article and Find Full Text PDFJ Sci Food Agric
November 2024
School of Pharmacy, Lanzhou University, Lanzhou, China.
Background: Codonopsis pilosula var. modesta (CPVM) is a famous medicinal and edible plant of Campanulaceae. However, fresh CPVM roots (FCPVR) are prone to softening, browning and spoilage after concentrated harvesting in the main production area of Gansu Province, China in autumn, which poses great challenges to their large-scale storage and modern processing.
View Article and Find Full Text PDFFood Res Int
August 2024
Wuya College of Innovation, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address:
Synthetic preservatives are widely used in the food industry to control spoilage and growth of pathogenic microorganisms, inhibit lipid oxidation processes and extend the shelf life of food. However, synthetic preservatives have some side effects that can lead to poisoning, cancer and other degenerative diseases. With the improvement of living standards, people are developing safer natural preservatives to replace synthetic preservatives, including plant derived preservatives (polyphenols, essential oils, flavonoids), animal derived preservatives (lysozyme, antimicrobial peptide, chitosan) and microorganism derived preservatives (nisin, natamycin, ε-polylysine, phage).
View Article and Find Full Text PDFBiochim Biophys Acta Biomembr
October 2024
Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark. Electronic address:
Polyene macrolides are antifungal substances, which interact with cells in a sterol-dependent manner. While being widely used, their mode of action is poorly understood. Here, we employ ultraviolet-sensitive (UV) microscopy to show that the antifungal polyene natamycin binds to the yeast plasma membrane (PM) and causes permeation of propidium iodide into cells.
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