A genetic variant in the immune-related gene ERAP1 affects colorectal cancer prognosis.

Chin Med J (Engl)

Department of Epidemiology and Biostatistics, School of Public Health, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei 430071, China.

Published: February 2024

Background: Findings on the association of genetic factors and colorectal cancer (CRC) survival are limited and inconsistent, and revealing the mechanism underlying their prognostic roles is of great importance. This study aimed to explore the relationship between functional genetic variations and the prognosis of CRC and further reveal the possible mechanism.

Methods: We first systematically performed expression quantitative trait locus (eQTL) analysis using The Cancer Genome Atlas (TCGA) dataset. Then, the Kaplan-Meier analysis was used to filter out the survival-related eQTL target genes of CRC patients in two public datasets (TCGA and GSE39582 dataset from the Gene Expression Omnibus database). The seven most potentially functional eQTL single nucleotide polymorphisms (SNPs) associated with six survival-related eQTL target genes were genotyped in 907 Chinese CRC patients with clinical prognosis data. The regulatory mechanism of the survival-related SNP was further confirmed by functional experiments.

Results: The rs71630754 regulating the expression of endoplasmic reticulum aminopeptidase 1 ( ERAP1 ) was significantly associated with the prognosis of CRC (additive model, hazard ratio [HR]: 1.43, 95% confidence interval [CI]: 1.08-1.88, P = 0.012). The results of dual-luciferase reporter assay and electrophoretic mobility shift assay showed that the A allele of the rs71630754 could increase the binding of transcription factor 3 (TCF3) and subsequently reduce the expression of ERAP1 . The results of bioinformatic analysis showed that lower expression of ERAP1 could affect the tumor immune microenvironment and was significantly associated with severe survival outcomes.

Conclusion: The rs71630754 could influence the prognosis of CRC patients by regulating the expression of the immune-related gene ERAP1 .

Trial Registration: No. NCT00454519 ( https://clinicaltrials.gov/ ).

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10876254PMC
http://dx.doi.org/10.1097/CM9.0000000000002845DOI Listing

Publication Analysis

Top Keywords

prognosis crc
12
crc patients
12
immune-related gene
8
gene erap1
8
colorectal cancer
8
survival-related eqtl
8
eqtl target
8
target genes
8
regulating expression
8
expression erap1
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!