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First report of L1 cell adhesion molecule (L1CAM) in flat fish (Starry flounder): Molecular features and expression analysis. | LitMetric

First report of L1 cell adhesion molecule (L1CAM) in flat fish (Starry flounder): Molecular features and expression analysis.

Dev Comp Immunol

Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, Republic of Korea. Electronic address:

Published: December 2023

AI Article Synopsis

  • The L1 cell adhesion molecule (L1CAM) sequence from the starry flounder was obtained and verified through next-generation sequencing techniques.
  • The study found that PsL1CAM mRNA was expressed across various tissues in healthy starry flounders, with notable changes in expression observed after infections with viral hemorrhagic septicemia virus (VHSV) and Streptococcus parauberis PH0710.
  • In response to VHSV infection, PsL1CAM mRNA levels increased in most tissues but decreased in mucosal-associated lymphoid tissues; likewise, after S. parauberis PH0710 infection, expression generally rose except in the heart, suggesting PsL1CAM's role in the fish's immune

Article Abstract

In this study, the starry flounder L1 cell adhesion molecule (L1CAM) sequence was obtained using next-generation sequencing, and the integrity of the sequence was verified by cloning and sequencing. First, the amino acid sequence was predicted using the cDNA sequence, and the gene was then identified through multiple sequence alignment analysis with related sequences and phylogenetic analysis. Thus, homogeneity was confirmed. The expression level of PsL1CAM (Platichthys stellatus L1CAM) mRNA in healthy starry flounder was detected in all tissues used in the experiment, and tissue- and gene-specific expression levels were confirmed. In addition, as a result of mRNA expression analysis after artificial infection with viral hemorrhagic septicemia virus (VHSV) and Streptococcus parauberis PH0710, significant expression changes and characteristics were confirmed following infection with VHSV and S. parauberis PH0710. After artificial infection with VHSV, the expression level of PsL1CAM mRNA was significantly upregulated in almost all major tissues of the starry flounder, whereas it was significantly downregulated in mucosal-associated lymphoid tissues, such as the gills and intestine. Infection with S. parauberis PH0710 significantly upregulated the expression of PsL1CAM mRNA in almost all major tissues of the starry flounder, whereas it was significantly downregulated in the heart after infection. Our results indicate that PsL1CAM may be involved in the host immune response to starry flounders.

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Source
http://dx.doi.org/10.1016/j.dci.2023.105054DOI Listing

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