Complete De Novo Assembly of Endosymbiont of .

As an endosymbiont, exerts significant effects on the host, including on reproduction, immunity, and metabolism. However, the study of in Thysanopteran insects, such as flower thrips , remains limited. Here, we assembled a gap-free looped genome assembly of strain FI in a length of 1,463,884 bp (GC content 33.80%), using Nanopore long reads and Illumina short reads. The annotation of FI identified a total of 1838 protein-coding genes (including 85 pseudogenes), 3 ribosomal RNAs (rRNAs), 35 transfer RNAs (tRNAs), and 1 transfer-messenger RNA (tmRNA). Beyond this basic description, we identified mobile genetic elements, such as prophage and insertion sequences (ISs), which make up 17% of the entire FI genome, as well as genes involved in riboflavin and biotin synthesis and metabolism. This research lays the foundation for understanding the nutritional mutualism between and flower thrips. It also serves as a valuable resource for future studies delving into the intricate interactions between and its host.