AI Article Synopsis
- The last two decades have seen rapid advancements in biomedical research focused on studying specific cell types and individual cells, particularly regarding their regulatory and transcriptional features.
- Researchers have been using next-generation sequencing (NGS) to investigate factors like chromatin accessibility and histone modifications in cells.
- The study combines techniques like FACS/FANS with ATAC-seq, ChIP-seq, and RNA-seq to analyze the unique chromatin and transcriptional profiles of different cell types.
Article Abstract
The past two decades in biomedical research have experienced an explosion of cell type-specific and single-cell studies, especially concerning the concomitant dissection of regulatory and transcriptional landscapes of those under investigation. Additionally, leveraging next-generation sequencing (NGS) platforms efforts have been undertaken to evaluate the effects of chromatin accessibility, histone modifications, or even transcription factor binding sites. We have shown that Fluorescence-Activated Nuclear Sorting (FANS) is an effective means to characterize the transcriptomes of nuclei from different tissues. In light of our own technical and experimental developments, we extend this effort to combine FACS/FANS with Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq), Chromatin Immunoprecipitation sequencing (ChIP-seq), and RNA sequencing (RNA-seq) for profiling individual cell types according to their chromatin and transcriptional states.
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| http://dx.doi.org/10.1007/978-1-0716-3354-0_5 | DOI Listing |
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