To understand force generation under a wide range of loads, the stepping of single kinesin molecules was measured at loads from -20 to 42 pN by optical tweezers with high temporal resolution. The optical trap has been improved to halve positional noise and increase bandwidth by using 200-nm beads. The step size of the forward and backward steps was 8.2 nm even over a wide range of loads. Histograms of the dwell times of backward steps and detachment fit well to two independent exponential equations with fast (~0.4 ms) and slow (>3 ms) time constants, indicating the existence of a fast step in addition to the conventional slow step. The dwell times of the fast steps were almost independent of the load and ATP concentration, while those of the slow backward steps and detachment depended on those. We constructed the kinetic model to explain the fast and slow steps under a wide range of loads.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/tra.12909 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cross-cultural adaptation and validation of a teaching questionnaire measuring facilitator competencies and characteristics of interprofessional clinical educators in an Asian setting.
J Interprof Care
January 2025
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia- Dr Cipto Mangunkusumo National Central Public Hospital, Jakarta, Indonesia.
This study aimed to validate an Indonesian version of the teaching questionnaire measuring the competencies of interprofessional education (IPE) facilitators and the characteristics of good clinical educators described by Kerry et al. (2021). A cross-cultural adaptation was developed and consisted of the following steps: forward-backward translation, content validity index measurement, cognitive interviews and a pilot study to measure content validity and reliability, exploratory factor analysis (EFA) to identify the new dimensionality, and confirmatory factor analysis (CFA) to confirm the measurement model.
View Article and Find Full Text PDFPhosphate rebinding induces force reversal via slow backward cycling of cross-bridges.
Front Physiol
January 2025
Institute of Vegetative Physiology, University of Cologne, Köln, Germany.
Objective: Previous studies on muscle fibers, myofibrils, and myosin revealed that the release of inorganic phosphate (P) and the force-generating step(s) are reversible, with cross-bridges also cycling backward through these steps by reversing force-generating steps and rebinding P. The aim was to explore the significance of force redevelopment kinetics (rate constant ) in cardiac myofibrils for the coupling between the P binding induced force reversal and the rate-limiting transition for backward cycling of cross-bridges from force-generating to non-force-generating states.
Methods: and force generation of cardiac myofibrils from guinea pigs were investigated at 0.
Translation, validation, and reliability of the Modified Occupational Questionnaire into Bahasa Melayu among older persons in Malaysia.
Aust Occup Ther J
February 2025
Centre for Rehabilitation and Special Needs Studies, Occupational Therapy Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, 50300, Malaysia.
Introduction: A Modified Occupational Questionnaire (MOQ) is a self-report questionnaire that records the occupational engagement of an individual hourly, focusing on activity category, reason for doing the activity, value to self, and value to society. Understanding the patterns of occupational engagement and meaningful time use in older persons is crucial for predicting physical and psychological health and establishing occupation-based interventions to support healthy aging. However, the MOQ, originally developed in an English-speaking country and may potentially be less suitable for Malaysian older persons, who are predominantly Malay-speaking and have distinct cultural adaptations.
View Article and Find Full Text PDFTranslation and assessment of encultured meaning of the Multi-Dimensional Scale of Perceived Social Support in Diné bizaad (Navajo) using community-based participatory action research methods.
Rural Remote Health
January 2025
Indiana University School of Medicine, Indianapolis, Indiana, USA.
Introduction: Perceived social support is a psychological construct that is used to describe the 'perception of adequacy' of the support being provided by a person's social network. Higher perceived social support has been linked to multiple benefits across numerous studies over the past several decades and among multiple populations. The Multidimensional Scale of Perceived Social Support (MSPSS) is a 12-item scale to assess the construct of perceived social support.
View Article and Find Full Text PDFNeuroplasticity in Diffuse Low-grade Gliomas: Backward Modelling of Brain-tumor Interactions Prior to Diagnosis is Needed to Better Predict Recovery after Treatment.
Curr Neurol Neurosci Rep
January 2025
Department of Neurosurgery, Gui de Chauliac Hospital, Montpellier University Medical Center, 80 Avenue Augustin Fliche, Montpellier, 34295, France.
Purpose Of Review: In low-grade glioma (LGG), besides the patient's neurological status and tumor characteristics on neuroimaging, current treatment guidelines mainly rely on the glioma's genetics at diagnosis to define therapeutic strategy, usually starting with surgical resection. However, this snapshot in time does not take into account the antecedent period of tumor progression and its interactions with the brain before presentation. This article reviews new concepts that pertain to reconstruct the history of previous interplay between the LGG's course and adaptive changes in the connectome within which the glioma is embedded over the years preceding the diagnosis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!