AI Article Synopsis

  • HIV reservoirs persist in various tissues even during effective antiretroviral therapy, with significant differences observed between anatomical compartments and individuals.
  • Intact HIV genomes are primarily found in secondary lymphoid organs, such as the spleen and lymph nodes, comprising 2% and 25% of proviruses in the study participants.
  • Identical HIV genomes are frequently found across multiple tissues, indicating that infected cells can expand, migrate, and circulate among different anatomical sites.

Article Abstract

HIV persists in tissues during antiretroviral therapy (ART), but the relative contribution of different anatomical compartments to the viral reservoir in humans remains unknown. We performed an extensive characterization of HIV reservoirs in two men who donated their bodies to HIV cure research and who had been on suppressive ART for years. HIV DNA is detected in all tissues, with large variations across anatomical compartments and between participants. Intact HIV genomes represent 2% and 25% of all proviruses in the two participants and are mainly detected in secondary lymphoid organs, with the spleen and mediastinal lymph nodes harboring intact viral genomes in both individuals. Multiple copies of identical HIV genomes are found in all tissues, indicating that clonal expansions are common in anatomical sites. The majority (>85%) of these expanded clones are shared across multiple tissues. These findings suggest that infected cells expand, migrate, and possibly circulate between anatomical sites.

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Source
http://dx.doi.org/10.1016/j.celrep.2023.113053DOI Listing

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