Probiotic Ln4 Showing Antimicrobial and Antibiofilm Effect against KCTC 5124 Causing Dental Caries.

Dental caries has known as an infectious disease that is considered a serious global public health problem. Recently, report indicate that probiotics play a vital role in maintaining oral health. Therefore, this study aimed to evaluate the prevention effects of Ln4 against dental infection by the pathogenic bacterium KCTC 5124 through biofilm formation inhibition. To evaluate such prevention effects against KCTC 5124, antimicrobial activity, auto-aggregation, co-aggregation, cell surface hydrophobicity, total exopolysaccharide (EPS) production rate, and biofilm formation were analyzed. Results showed that Ln4 showed higher antimicrobial activity than GG (LGG). In the group treated with Ln4, the co-aggregation (58.85%), cell surface hydrophobicity (16.75%), and EPS production rate (73.29%) values were lower than those of LGG and the negative control. Additionally, crystal violet staining and confocal laser scanning microscopy (CLSM) revealed that Ln4 effectively inhibited biofilm formation in KCTC 5124. Therefore, Ln4 could be used in the industry as a probiotics to prevent and improve oral health.