AI Article Synopsis
- Zebrafish scales are valuable for studying how body parts regenerate due to their unique properties and quick recovery time, which takes about 2 weeks after loss.
- Researchers can use advanced imaging techniques like live confocal microscopy to observe the regeneration process in real-time at both tissue-wide and sub-cellular levels.
- This study highlights methods for capturing and analyzing images that reveal how cells and signals behave during the regeneration of zebrafish scales.
Article Abstract
In regeneration, a damaged body part grows back to its original form. Understanding the mechanisms and physical principles underlying this process has been limited by the difficulties of visualizing cell signals and behaviors in regeneration. Zebrafish scales are emerging as a model system to investigate morphogenesis during vertebrate regeneration using quantitative live imaging. Scales are millimeter-sized dermal bone disks forming a skeletal armor on the body of the fish. The scale bone is deposited by an adjacent monolayer of osteoblasts that, after scale loss, regenerates in about 2 weeks. This intriguing regenerative process is accessible to live confocal microscopy, quantifications, and mathematical modeling. Here, I describe methods to image scale regeneration live, tissue-wide and at sub-cellular resolution. Furthermore, I describe methods to process the resulting images and quantify cell, tissue, and signal dynamics.
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| http://dx.doi.org/10.1007/978-1-0716-3401-1_12 | DOI Listing |
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