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Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine.
Eur J Clin Microbiol Infect Dis
November 2024
Department of Biotechnology, Inland Norway University of Applied Sciences, Holsetgata 22, Hamar, 2317, Norway.
Purpose: Urinary Tract Infections (UTIs) are among the most prevalent infections globally. Every year, approximately 150 million people are diagnosed with UTIs worldwide. The current state-of-the-art diagnostic methods are culture-based and have a turnaround time of 2-4 days for pathogen identification and susceptibility testing.
View Article and Find Full Text PDFPurpose: To provide a descriptive report of mortality and morbidity in the first 30 days of diagnosis of urosepsis. Secondary aim is to identify risk factors of unfavourable outcomes.
Methods: Prospective observational multicentre cohort study conducted from September 2014 to November 2018 in European hospitals.
A reference standard for urinary tract infection research: a multidisciplinary Delphi consensus study.
Lancet Infect Dis
August 2024
Department of Infectious Diseases, Leiden University Medical Center, Leiden, Netherlands.
The absence of a consensus-based reference standard for urinary tract infection (UTI) research adversely affects the internal and external validity of diagnostic and therapeutic studies. This omission hinders the accumulation of evidence for a disease that imposes a substantial burden on patients and society, particularly in an era of increasing antimicrobial resistance. We did a three-round Delphi study involving an international, multidisciplinary panel of UTI experts (n=46) and achieved a high degree of consensus (94%) on the final reference standard.
View Article and Find Full Text PDFAntimicrobial Prophylaxis in Robot-Assisted Laparoscopic Radical Prostatectomy: A Systematic Review.
Antibiotics (Basel)
December 2023
Department of Urology, University College London Hospitals, London NW1 2BU, UK.
Article Synopsis
- The study examines whether antibiotic prophylaxis (AP) is beneficial during robot-assisted laparoscopic radical prostatectomy (RALP) for prostate cancer, highlighting concerns over antibiotic resistance and side effects.
- A systematic literature review reviewed 436 publications, ultimately finding 8 relevant studies involving 6,378 RALP procedures, yet no substantial evidence showed effective differences in infection rates based on varying AP protocols.
- Overall, infection rates post-surgery ranged from 0.6% to 6.6%, but the lack of scientific proof for AP's efficacy suggests a need for more uniform research to provide better guidance on its use in RALP.
Antibiotic Resistance Patterns Among Uropathogens in Female Outpatients Affected by Uncomplicated Cystitis: Focus on Fosfomycin Trometamol.
Int J Antimicrob Agents
November 2023
Department of Urology, University of Naples, Federico II, Naples, Italy.
Objectives: To report the resistance rate against fosfomycin trometamol among outpatient women with symptoms related to urinary tract infections over a 6-year period in a multicentre, cross-sectional study.
Methods: Urinary samples were collected from three high-volume laboratories from January 2015 to December 2020. The pattern of resistance to fosfomycin was analysed by using the Vitek II automated system.
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