LncRNA MACC1-AS1 associates with DDX5 to modulate transcription in breast cancer cells.

is a master oncogene involved in multiple aspects of cancer metastasis in a broad variety of tumors. However, the molecular mechanism by which transcription is regulated remains unclear. Here, we show that in breast cancer cells, lncRNA MACC1-AS1 serves as a -factor to up-regulate transcription and this regulation increases the cell proliferation potential. Mechanistically, MACC1-AS1 forms a complex with DEAD-Box helicase 5 (DDX5) and simultaneously interacts with the distal region of the promoter. The interaction allows its associated DDX5 to spatially contact the core promoter and shift from MACC1-AS1 to the core promoter. Moreover, binding of DDX5 to the core promoter results in local recruitment of the transcription factor SP-1, thus enhancing transcription. Our findings reveal a molecular mechanism by which MACC1-AS1 -regulates transcription by interacting with the distal promoter region and delivering DDX5 to the core-promoter of the gene.