High-throughput transcriptome sequencing reveals the protective role of adenosine receptor-related genes in paraquat-exposed .

Toxicol Res (Camb)

College of Life Sciences, Engineering Training Centre/College of Innovation, China Jiliang University, Hangzhou 310018, China.

Published: August 2023

AI Article Synopsis

  • This study analyzed the protective role of adenosine against oxidative stress caused by paraquat (PQ) through its receptor, ADOR-1, by comparing treated and untreated groups.
  • Samples were sequenced to identify gene expression changes, revealing that several genes were differently expressed in response to adenosine, linked to neuropeptide and cAMP pathways.
  • The findings suggest that certain G-protein-coupled receptors are key in adenosine's protective effects, with consistent expression of the ADOR-1 gene across all treatments, pointing to a stable pathway influence despite PQ exposure.

Article Abstract

This study sought to identify the genes associated with adenosine's protective action against paraquat (PQ)-induced oxidative stress via the adenosine receptor (ADOR-1) in (). The was divided into 3 groups-2 groups exposed to PQ, one in presence, and one in absence of adenosine-and a control group that was not treated. Each group's total RNA was extracted and sequenced. When the transcriptomes of these groups were analyzed, several genes were found to be differently expressed. These differentially expressed genes were significantly enriched in adenosine-response biological processes and pathways, including gene ontology terms related to neuropeptide and kyoto encyclopedia of genes and genomes pathways associated to cAMP pathway regulator activity. Quantitative reverse-transcription PCR confirmed that G-protein-coupled receptors signaling pathway involving , , , , and genes may play crucial roles in modulating adenosine's protective action. Interestingly, there are no significant variations in the expression of the gene across the 3 treatments, thereby indicating that adenosine receptor exerts a consistent and stable influence on its related pathways irrespective of the presence or absence of PQ. Furthermore, the wild-type group with gene has higher survival rate than that of the /RNA interference group while treated with PQ in the presence of adenosine. Conclusively, our study uncovered a number of novel PQ-response genes and adenosine receptor-related genes in , which may function as major regulators of PQ-induced oxidative stress and indicate the possible protective effects of adenosine.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470339PMC
http://dx.doi.org/10.1093/toxres/tfad046DOI Listing

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