Investigating the efficacy of topical application of herbal cream in preventing skin damage induced by UVB radiation in a rat model.

Ultraviolet-B irradiation is a common environmental stressor that has detrimental effects on human skin. Natural sunscreens are well-known for their ability to benefit inflamed sunburn and dry skin. This study examined the effect of formulated herbal cream on UVB-induced skin damage. We screened the bioactive compounds of crude extract, showing significant antioxidant activity. Additionally, we evaluated the cytotoxicity, revealing that extract has less toxicity to vero cells (IC 98.45 μg/mL) than to A375 cells (IC 48.95 μg/mL). Based on this, we formulated the herbal cream () at 50, 100 and 200 mg concentrations and evaluated its organoleptic characteristics. Then, the rats were exposed to UVB radiation (32,800 J/m) four times/week (on alternate days) before the cream was applied topically to the dorsal skin surface. Under UVB stress without treatment, rats showed deep dermal damage. In contrast, rats treated with the exhibited significantly reduced sunburn. Moreover, the histopathological and biochemical assays were confirmed by the topical application of , which had potentially reduced the skin elasticity and maintained the imbalanced enzyme and non-enzymatic antioxidant activity. Our findings amply demonstrate that the significantly accelerated the recovery of UVB-induced lesions through antioxidant and down-regulation of skin photodamage.