AI Article Synopsis
- Fe-TAML is a new small molecule probe that acts as a peroxidase, enhancing imaging capabilities in correlated fluorescence and electron microscopy.
- It enables high-resolution imaging of new DNA, genetically tagged proteins, and untagged endogenous proteins through various labeling techniques.
- The probe's effectiveness comes from its ability to create visible deposits using its catalytic activity, making it a valuable tool for studying biomolecular species in cellular environments at nano and micro scales.
Article Abstract
We introduce Fe-TAML, a small molecule-based peroxidase as a versatile new member of the correlated fluorescence and electron microscopy toolkit. The utility of the probe is demonstrated by high resolution imaging of newly synthesized DNA (through biorthogonal labeling), genetically tagged proteins (using HaloTag), and untagged endogenous proteins (via immunostaining). EM visualization in these applications is facilitated by exploiting Fe-TAML's catalytic activity for the deposition of localized osmiophilic precipitates based on polymerized 3,3'-diaminobenzidine. Optimized conditions for synthesizing and implementing Fe-TAML based probes are also described. Overall, Fe-TAML is a new chemical biology tool that can be used to visualize diverse biomolecular species along nanometer and micron scales within cells.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473768 | PMC |
| http://dx.doi.org/10.1101/2023.08.25.554352 | DOI Listing |
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