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Measuring calcium content in plants using NEXAFS spectroscopy. | LitMetric

AI Article Synopsis

  • * Traditional methods for measuring calcium, like AAS and ICP-MS, require large samples and chemical processing, limiting their use.
  • * The study introduces NEXAFS spectroscopy as a new technique to quantify calcium content in plant samples with better spatial resolution and without chemical extraction, validated using onion peels and model plant hypocotyls.

Article Abstract

Calcium is important for the growth and development of plants. It serves crucial functions in cell wall and cell membrane structure and serves as a secondary messenger in signaling pathways relevant to nutrient and immunity responses. Thus, measuring calcium levels in plants is important for studies of plant biology and for technology development in food, agriculture, energy, and forest industries. Often, calcium in plants has been measured through techniques such as atomic absorption spectrophotometry (AAS), inductively coupled plasma-mass spectrometry (ICP-MS), and electrophysiology. These techniques, however, require large sample sizes, chemical extraction of samples or have limited spatial resolution. Here, we used near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at the calcium L- and K-edges to measure the calcium to carbon mass ratio with spatial resolution in plant samples without requiring chemical extraction or large sample sizes. We demonstrate that the integrated absorbance at the calcium L-edge and the edge jump in the fluorescence yield at the calcium K-edge can be used to quantify the calcium content as the calcium mass fraction, and validate this approach with onion epidermal peels and ICP-MS. We also used NEXAFS to estimate the calcium mass ratio in hypocotyls of a model plant, , which has a cell wall composition that is similar to that of onion epidermal peels. These results show that NEXAFS spectroscopy performed at the calcium edge provides an approach to quantify calcium levels within plants, which is crucial for understanding plant physiology and advancing plant-based materials.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468975PMC
http://dx.doi.org/10.3389/fpls.2023.1212126DOI Listing

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